matrigel-coated transwell chambers  (Corning Life Sciences)

Journal: Materials Today Bio

Article Title: A MnO 2 -based tumor-seeking nanoplatform for enhanced chemoimmunotherapy against 4T1 breast cancer

doi: 10.1016/j.mtbio.2025.102000

Figure Lengend Snippet: Cellular uptake of Squ@APS NPs and Squ@APS-IR820 NPs and transwell invasion assays. Fluorescence image of 4T1 cells co-cultured with Squ/C6@APS (A) and Squ/C6@APS-IR820 (B) at 1 h, 3 h, and 6 h, respectively. Blue and green represent DAPI and C6 fluorescence, which stand for the position of cell nucleus and NPs. The endocytosis of Squ/C6@APS (C) and Squ/C6@APS-IR820 (D) by 4T1 cells was visualized by flow cytometry. Blank represents cells treated with the same volume of medium, which was used as a control (Red). The mean fluorescence intensity (MFI) value (E) of 4T1 cells co-cultured with Squ/C6@APS and Squ/C6@APS-IR820NPs for 1 h, 3 h, and 6 h. Fluorescence image (F) and the mean fluorescence intensity (MFI) value (G) of 4T1 cells co-cultured with Squ/C6@APS-IR820 NPs for 3 h pretreated with 100 μM of probenecid or 50 μM of doxorubicin for 30 min. Invasion of 4T1 cells treated with saline (H), Squ@APS-IR820 NPs (I), MnO 2 @APS-IR820 (J), and Squ@APS-IR820 NPs + MnO 2 @APS-IR820 (K). Invasive cells in every field (L). Data are presented as means ± standard deviation (SD). Differences among groups were evaluated by one-way ANOVA analysis. ∗∗∗ p < 0.001, ∗∗ p < 0.01, and ∗ p < 0.05. ns, no significant difference. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Article Snippet: The invasive capacity of 4T1 cells was assessed using matrigel-coated transwell chambers (6.5 mm diameter, 8 μm pore size; Corning).

Techniques: Fluorescence, Cell Culture, Flow Cytometry, Control, Saline, Standard Deviation